Matrices listed below were spiked with certain level of IVL and the recovery rates were calculated by comparing the measured value to the expected amount of IVL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-98
94
EDTA plasma(n=5)
85-103
93
heparin plasma(n=5)
92-104
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IVL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IVL ELISA Kit allows for the in vitro quantitative determination of IVL concentrations in serum, plasma, tissue homogenates and other biological fluids.