Human ITIH3(Inter Alpha-Globulin Inhibitor H3) ELISA Kit

Mfr.No.

ELK3565

Sensitivity

2.39 ng/mL

Detection Range

6.25-400 ng/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

H3P; SHAP; Pre-Alpha(Globulin)Inhibitor,H3 Polypeptide; Serum-derived hyaluronan-associated protein

Recovery

Matrices listed below were spiked with certain level of recombinant ITIH3 and the recovery rates were calculated by comparing the measured value to the expected amount of ITIH3 in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	80-95%	87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ITIH3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	80-101%	87-96%	96-105%	95-103%
EDTA plasma(n=5)	95-104%	82-96%	92-105%	83-92%
Heparin plasma(n=5)	86-93%	91-105%	87-99%	99-105%

Typical Data

<table> <tbody><tr> <th>Concentration (ng/mL)</th> <th>OD</th> <th>Corrected OD</th> </tr> <tr> <td>400.00</td> <td>2.075</td> <td>1.994</td> </tr> <tr> <td>200.00</td> <td>1.647</td> <td>1.566</td> </tr> <tr> <td>100.00</td> <td>1.240</td> <td>1.159</td> </tr> <tr> <td>50.00</td> <td>0.882</td> <td>0.801</td> </tr> <tr> <td>25.00</td> <td>0.492</td> <td>0.411</td> </tr> <tr> <td>12.50</td> <td>0.389</td> <td>0.308</td> </tr> <tr> <td>6.25</td> <td>0.226</td> <td>0.145</td> </tr> <tr> <td>0.00</td> <td>0.081</td> <td>0.000</td> </tr> </tbody></table>

Limit Of Detection

400 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITIH3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITIH3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITIH3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITIH3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

<a href="https://s3.us-west-1.amazonaws.com/s3.biohippo.com/elisakits/elk/ELK3565-1.pdf" target="_blank">Datasheet</a>

Applications	ELISA
Research Area	Metabolic pathway;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15202812	ELK3565	48T	$320		Add to Cart
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Human ITIH3(Inter Alpha-Globulin Inhibitor H3) ELISA Kit

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