Human ITGa1(Integrin Alpha 1) ELISA Kit

Mfr.No.

ELK4029

Alternative Names

CD49-A; CD49a; ITG-A1; VLA1; CD49 antigen-like family member A; Laminin and collagen receptor

Reactivity

Human

Detection Range

78.13-5000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant ITGa1 and the recovery rates were calculated by comparing the measured value to the expected amount of ITGa1 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	86-99%	92%
Heparin plasma(n=5)	83-98%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ITGa1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	87-98%	92-104%	82-96%	95-102%
EDTA plasma(n=5)	89-97%	85-94%	79-96%	85-99%
Heparin plasma(n=5)	86-97%	83-96%	91-103%	96-105%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.266	2.198
2500.00	1.566	1.498
1250.00	1.217	1.149
625.00	0.992	0.924
312.50	0.567	0.499
156.25	0.256	0.188
78.13	0.196	0.128
0.00	0.068	0.000

Sensitivity

29 pg/mL

Limit Of Detection

5000 pg/mL

UniProt ID	P56199
Antigen	ITGa1
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGa1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITGa1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	CD & Adhesion molecule;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15202433	ELK4029	48T	$320		Add to Cart
BHE15202433	ELK4029	96T	$458		Add to Cart

Human ITGa1(Integrin Alpha 1) ELISA Kit

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