Human ITaC(Interferon Inducible T-Cell Alpha Chemoattractant) ELISA Kit

Mfr.No.

ELK1344

Alternative Names

CXCL11; I-TAC; IP9; SCYB11; SCYB9B; B-R1; Chemokine(C-X-C-Motif)ligand 11; Small Inducible Cytokine Subfamily B Member 11; Interferon gamma-inducible protein 9

Reactivity

Human

Detection Range

62.5-4000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant ITaC and the recovery rates were calculated by comparing the measured value to the expected amount of ITaC in samples.

Matrix	Recovery range	Average
serum(n=5)	82-94%	88%
EDTA plasma(n=5)	94-105%	98%
Heparin plasma(n=5)	83-97%	91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ITaC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	95-103%	91-105%	78-90%	89-97%
EDTA plasma(n=5)	85-94%	87-96%	82-93%	89-103%
Heparin plasma(n=5)	85-92%	83-101%	87-98%	79-92%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
4000.00	1.954	1.867
2000.00	1.653	1.566
1000.00	1.276	1.189
500.00	0.943	0.856
250.00	0.623	0.536
125.00	0.354	0.267
62.50	0.227	0.140
0.00	0.087	0.000

Sensitivity

24.2 pg/mL

Limit Of Detection

4000 pg/mL

UniProt ID	O14625
Antigen	ITaC
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITaC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITaC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITaC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITaC in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Cytokine;Signal transduction;Infection immunity;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15204086	ELK1344	48T	$265		Add to Cart
BHE15204086	ELK1344	96T	$376		Add to Cart

Human ITaC(Interferon Inducible T-Cell Alpha Chemoattractant) ELISA Kit

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