Human Isodesmosine (IDES) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14052h

Sensitivity

11.75 pg/mL

Detection Range

47 pg/mL-3000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Isodesmosine (IDES)

Recovery

The recovery of human DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-98
EDTA plasma (n=4)	94	89-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	90
	Range %	86-94
1:4	Average %	90
	Range %	86-94
1:8	Average %	102
	Range %	97-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IDES

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Procollagen III N-terminal propeptide and desmosine are released by matrix destruction in pulmonary tuberculosis Seddon J et al,J Infect Dis,2013 Aug 9.

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