Human Isocitrate Dehydrogenase - Nadp - Mitochondrial

Mfr.No.

E3098Hu

Sensitivity

0.3ng/ml

Detection Range

0.5-300ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Isocitrate Dehydrogenase 2 (also known as IDH2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

D2HGA2;ICD-M;IDH;IDH2;IDHM;IDP;IDPM;Isocitrate dehydrogenase (NADP), mitochondrial;NADP(+-specific ICDH;Oxalosuccinate decarboxylase

Molecular Weight

50909

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3098Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	50.81	2.48	4.88
Intra-Assay	2	18	139.62	4.25	3.05
Intra-Assay	3	18	62.17	1.94	3.13

Background

Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.

Protocol

These standard curves of E3098Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160ng/ml	1.857	1.919	1.859
	1.981
80ng/ml	0.908	0.974	0.914
	1.04
40ng/ml	0.572	0.593	0.533
	0.613
20ng/ml	0.149	0.198	0.138
	0.246
10ng/ml	0.089	0.096	0.036
	0.102
0ng/ml	0.059	0.06	0
	0.061

Gene ID	3418
UniProt ID	P48735

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104631	E3098Hu	48T	$320		Add to Cart
BHE12104631	E3098Hu	96T	$458		Add to Cart

Human Isocitrate Dehydrogenase - Nadp - Mitochondrial - IDH2 ELISA Kit

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