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Home / ELISA Kit

Human islet cell antibody,ICA ELISA Kit

SKU : BHE10503035

This Human AMY1 ELISA Kit was designed for the quantitative measurement of Human AMY1 protein in serum, saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 IU/mL-500 IU/mL and the sensitivity is 1.95 IU/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E04972h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

islet cell antibody,ICA

Recovery

The recovery of human AMY1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		100		96-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AMY1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		88
		Range %		81-93
1:2		Average %		92
		Range %		89-96
1:4		Average %		90
		Range %		84-96
1:8		Average %		93
		Range %		86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

IU/ml	OD1	OD2	Average	Corrected
500	2.991	2.789	2.890	2.788
250	2.607	2.604	2.606	2.504
125	2.254	2.213	2.234	2.132
62.5	1.682	1.569	1.626	1.524
31.2	0.931	0.924	0.928	0.826
15.6	0.522	0.513	0.518	0.416
7.8	0.291	0.288	0.290	0.188
0	0.106	0.098	0.102	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

Applications	ELISA
Research Area	ICA

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10503035	CSB-E04972h	96T	$495		Add to Cart
BHE10503035	CSB-E04972h	5x96T	$2376		Add to Cart
BHE10503035	CSB-E04972h	10x96T	$4702.50		Add to Cart

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