Human islet amyloid polypeptide,IAPP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09624h

Alternative Names

islet amyloid polypeptide, AMYLIN, DAP, IAP, Islet amyloid polypeptide (diabetes-associated peptide; amylin)

Reactivity

Human

Detection Range

47 pg/mL-3000 pg/mL

Sample Type

Serum, Plasma, Urine

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-92
EDTA plasma (n=4)	89	85-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	95
	Range %	89-99
1:4	Average %	90
	Range %	86-94
1:8	Average %	102
	Range %	98-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

11.75 pg/mL

Limit Of Detection

450 nm

UniProt ID	P10997
Antigen	Islet Amyloid Polypeptide

Applications

ELISA

Research Area

IAPP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503033	CSB-E09624h	96T	$790	Add to Cart
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BHE10503033	CSB-E09624h	10x96T	$7505	Add to Cart