Human Ischemia Modified Albumin,IMA ELISA Kit

Mfr.No.

CSB-E09594h

Sensitivity

0.78 IU/mL

Detection Range

3.12 IU/mL-200 IU/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Ischemia Modified Albumin,IMA

Recovery

The recovery of human TGM4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-104
EDTA plasma (n=4)	93	88-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TGM4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-97
1:2	Average %	90
	Range %	83-100
1:4	Average %	95
	Range %	89-110
1:8	Average %	95
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	IMA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The protective effect of tadalafil on IMA (ischemia modified albumin) levels in experimental renal ischemia-reperfusion injury Amasyali AS. et al,Int J Clin Exp Med,8(9):15766-15772. Ischemia-modified albumin as an oxidative stress biomarker in early pregnancy loss Cengiz H. et al,J Matern Fetal Neonatal Med.,2015 Sep 18:1-4. Effect of Ventilation Support on Oxidative Stress and Ischemia-Modified Albumin in Neonates. Dursun A. et al,Am J Perinatol,2015 Aug 24. Ursolic acid enhances pentobarbital-induced sleeping behaviors via GABAergic neurotransmission in mice Jeon SJ. et al,Eur J Pharmacol,2015 Sep 5;762:443-8. Albumin asMarker for susceptibility to metal ions in metal-on-metal hip prosthesis patients Facchin F.et al,Hum Exp Toxicol.,/ ISCHEMIA MODIFIED ALBUMIN IN PERINATOLOGY Gursoy.et al,European Journal of Obstetrics & Gynecology and Reproductive Biology,/ Ischemic modified albumin increases in acute kidney injury Mertoglu, Cuma.et al,Revista Romana de Medicina de Laborator ,Jan 30 Stronger correlation with myocardial ischemia of high-sensitivity troponin T than other biomarkers Pipikos T.et al,J Nucl Cardiol,Jan 29 Acute Responses of Novel Cardiac Biomarkers to a 24-h Ultra-Marathon Zebrowska A, et al,Journal of Clinical Medicine,January 8

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