Matrices listed below were spiked with certain level of IS and the recovery rates were calculated by comparing the measured value to the expected amount of IS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-103
96
EDTA plasma(n=5)
87-105
95
heparin plasma(n=5)
91-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
87-100%
85-94%
EDTA plasma(n=5)
89-101%
87-96%
86-99%
heparin plasma(n=5)
85-94%
86-97%
89-95%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
IS ELISA Kit allows for the in vitro quantitative determination of IS concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Human IS (serum indoxyl sulfate) ELISA Kit