Matrices listed below were spiked with certain level of IRS2 and the recovery rates were calculated by comparing the measured value to the expected amount of IRS2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
93
EDTA plasma(n=5)
86-105
99
heparin plasma(n=5)
85-102
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IRS2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IRS2 ELISA Kit allows for the in vitro quantitative determination of IRS2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human IRS2(Insulin receptor substRate 2) ELISA Kit