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Home / ELISA Kit

Human Intestinal-type Alkaline Phosphatase - ALPI ELISA Kit

SKU : BHE12106890

Alkaline phosphatase, intestinal also known as ALPI is a type of alkaline phosphatase that in humans is encoded by the ALPI gene.

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Mfr.No.

E5528Hu

Sensitivity

0.55ng/mL

Detection Range

1-400ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ALPI;IAP;Intestinal alkaline phosphatase;Intestinal-type alkaline phosphatase

Molecular Weight

56812

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5528Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	159.01	4.73	2.97
Intra-Assay	2	18	107.12	3.68	3.44
Intra-Assay	3	18	80.91	3.54	4.38

Background

Alkaline phosphatase, intestinal also known as ALPI is a type of alkaline phosphatase that in humans is encoded by the ALPI gene.

Protocol

These standard curves of E5528Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/mL	2.159	1.985	1.926
	1.811
120ng/mL	1.076	1	0.941
	0.924
60ng/mL	0.445	0.48	0.421
	0.516
30ng/mL	0.284	0.226	0.167
	0.167
15ng/mL	0.167	0.125	0.066
	0.082
0ng/mL	0.054	0.059	0
	0.064

Gene ID	248
UniProt ID	P09923

Research Area	Cell Biology; Tags & Cell Markers

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106890	E5528Hu	48T	$320		Add to Cart
BHE12106890	E5528Hu	96T	$458		Add to Cart

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