Human Interleukin 9,IL-9 ELISA KIT

Mfr.No.

CSB-E04642h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

interleukin 9, HP40, IL-9, P40, T-cell growth factor p40, homolog of mouse T cell and mast cell growth factor 40, p40 T-cell and mast cell growth factor, p40 cytokine

Recovery

The recovery of Guinea pig T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-99
EDTA plasma (n=4)	93	85-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Guinea pig T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-100
1:2	Average %	90
	Range %	81-99
1:4	Average %	92
	Range %	85-100
1:8	Average %	90
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	?
320	0.175	0.171	0.173	?
160	0.275	0.280	0.278	?
80	0.502	0.493	0.498	?
40	1.006	1.028	1.017	?
20	2.024	2.049	2.037	?

Species

Homo sapiens

Sample Type

Serum, Cell culture supeatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P15248

Applications	ELISA
Research Area	IL9

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503024	CSB-E04642h	96T	$395	Add to Cart
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BHE10503024	CSB-E04642h	10x96T	$3752.50	Add to Cart

Human Interleukin 9,IL-9 ELISA KIT

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