Human interleukin 8(IL8) autoantibody ELISA kit

Human interleukin 8(IL8) autoantibody ELISA kit

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Mfr.No.

CSB-EQ027463HU

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

interleukin 8(IL8) autoantibody, autoantibody for CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, T cell chemotactic factor, beta-thromboglobulin-like protein, chemokine (C-X-C motif) ligand 8, emoctakin, granulocyte chemotactic

Recovery

The recovery of human GM2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-103
EDTA plasma (n=4)	90	84-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GM2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	92
	Range %	87-99
1:4	Average %	100
	Range %	95-106
1:8	Average %	97
	Range %	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.043	2.050	2.047	1.921
500	1.619	1.684	1.652	1.526
250	1.105	1.200	1.153	1.027
125	0.683	0.703	0.693	0.567
62.5	0.403	0.421	0.412	0.286
31.2	0.296	0.322	0.309	0.183
15.6	0.241	0.235	0.238	0.112
0	0.123	0.129	0.126	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IL8 auto-Ab

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503022	CSB-EQ027463HU	96T	$695	Add to Cart
BHE10503022	CSB-EQ027463HU	5x96T	$3336	Add to Cart
BHE10503022	CSB-EQ027463HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.