Human Interleukin 8 - IL-8 ELISA Kit

Mfr.No.

E0089Hu

Description

This sandwich kit is for the accurate quantitative detection of human Interleukin 8 (also known as IL-8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

(Ala-IL-877;(Ser-IL-872;Chemokine;C-X-C motif chemokine 8;C-X-C motif ligand 8;Emoctakin;GCP/IL-8 protein I;GCP/IL-8 protein II;GCP/IL-8 protein III;GCP/IL-8 protein IV;GCP/IL-8 protein V;GCP/IL-8 protein VI;GCP-1;Granulocyte chemotactic protein 1;IL 8;IL8;IL-8;IL-8(1-77;IL-8(5-77;IL-8(6-77;IL-8(7-77;IL-8(8-77;IL-8(9-77;IL8/NAP1 form I;IL8/NAP1 form II;IL8/NAP1 form III;IL8/NAP1 form IV;IL8/NAP1 form V;IL8/NAP1 form VI;Interleukin-8;Lymphocyte-derived neutrophil-activating factor;LYNAP;MDNCF;MDNCF-a;MDNCF-b;MDNCF-c;MONAP;Monocyte-derived neutrophil chemotactic factor;NAF;NAP-1;Monocyte-derived neutrophil-activating Peptide;Neutrophil-activating factor;Neutrophil-activating protein 1;Protein 3-10C;T-cell chemotactic factor;

Reactivity

Human

Detection Range

5-1000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

11098

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.51ng/L

Background

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

Gene ID	3576
UniProt ID	P10145
Antigen	IL8

Research Area

Cardiovascular; Immunology; Cancer

Protocol

These standard curves of E0089Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.307	2.349	2.287
	2.39
320ng/L	1.251	1.321	1.26
	1.391
160ng/L	0.686	0.682	0.62
	0.678
80ng/L	0.4	0.402	0.341
	0.404
40ng/L	0.318	0.31	0.249
	0.303
0ng/L	0.045	0.061	0
	0.077

Precision

We measured random samples of E0089Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	134.2	8.57	6.4
Intra-Assay	2	18	276.4	16.51	6
Intra-Assay	3	18	400.5	12.96	3.2

Storage	2-8°C

Reference	A Single Vitamin D3 Bolus Supplementation Improves Vitamin D Status and Reduces Proinflammatory Cytokines in Healthy Females Journal: Nutrients. 2022 Sep 24;14(19):3963. Effects of Olive Leaf Extract on Metabolic Response, Liver and Kidney Functions and Inflammatory Biomarkers in Hypertensive Patients Journal: Pak J Biol Sci. 2019 Jan;22(7):342-348. Effect of IL-6, IL-8/CXCL8, IP-10/CXCL 10 levels on the severity in COVID 19 infection Journal: Int J Clin Pract. 2021 Dec;75(12):e14970. Evaluation of periodontal status and cytokine response in children with familial Mediterranean fever or systemic juvenile idiopathic arthritis Journal: Clin Oral Investig. 2023 Mar;27(3):1159-1166. Comparison of Two Different Inhalation Anesthetics on Grafted Kidney Function in Patients Undergoing Renal Transplantation Surgery: Desflurane or Sevoflurane? Journal: Transplant Proc. 2017 Apr;49(3):448-453.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101824	E0089Hu	96T	$458		Add to Cart
BHE12101824	E0089Hu	48T	$320		Add to Cart

Human Interleukin 8 - IL-8 ELISA Kit

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