Human Interleukin 6 receptor(IL-6R)ELISA Kit

Human Interleukin 6 receptor(IL-6R)ELISA Kit

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Mfr.No.

CSB-E16524h

Alternative Names

interleukin 6 receptor, CD126, IL-6R-1, IL-6R-alpha, IL6RA, MGC104991, CD126 antigen, interleukin 6 receptor alpha subunit

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PROZ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	86-104
EDTA plasma (n=4)	95	87-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PROZ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	89-107
1:2	Average %	90
	Range %	87-103
1:4	Average %	89
	Range %	82-98
1:8	Average %	93
	Range %	87-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
8000	2.601	2.525	2.563	2.425
4000	1.882	1.910	1.896	1.758
2000	1.175	1.183	1.179	1.041
1000	0.658	0.682	0.670	0.532
500	0.364	0.371	0.368	0.230
250	0.249	0.260	0.255	0.117
125	0.208	0.211	0.210	0.072
0	0.139	0.137	0.138

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	P08887
Antigen	Interleukin 6 Receptor

Applications

ELISA

Research Area

IL6R

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of alcohol on diethylnitrosamine-induced hepatic toxicity: Critical role of ROS, lipid accumulation, and mitochondrial dysfunction Sun Q. et al,Exp Toxicol Pathol,2015 Jul 18.pii: S0940-2993(15)30007-5.

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SKU	Supplier No.	Size	Your price
BHE10503020	CSB-E16524h	96T	$695	Add to Cart
BHE10503020	CSB-E16524h	5x96T	$3336	Add to Cart
BHE10503020	CSB-E16524h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.