Human Interleukin 6 receptor(IL-6R)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E16524h

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

interleukin 6 receptor, CD126, IL-6R-1, IL-6R-alpha, IL6RA, MGC104991, CD126 antigen, interleukin 6 receptor alpha subunit

Recovery

The recovery of human PROZ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	86-104
EDTA plasma (n=4)	95	87-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PROZ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	89-107
1:2	Average %	90
	Range %	87-103
1:4	Average %	89
	Range %	82-98
1:8	Average %	93
	Range %	87-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P08887

Applications	ELISA
Research Area	IL6R

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of alcohol on diethylnitrosamine-induced hepatic toxicity: Critical role of ROS, lipid accumulation, and mitochondrial dysfunction Sun Q. et al,Exp Toxicol Pathol,2015 Jul 18.pii: S0940-2993(15)30007-5.

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