Human Interleukin 37(IL-37) ELISA Kit

Human Interleukin 37(IL-37) ELISA Kit

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Mfr.No.

CSB-E16185h

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

interleukin 1 family, member 7 (zeta), FIL1, FIL1(ZETA), FIL1Z, IL-1F7, IL-1H4, IL-1RP1, IL1H4, IL1RP1, IL-1F7b (IL-1H4, IL-1H, IL-1RP1), IL-1X protein, IL1F7 (canonical product IL-1F7b), interleukin 1 family member 7, interleukin 1 family,

Recovery

The recovery of rat LGALS3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-91
EDTA plasma (n=4)	87	83-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat LGALS3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-93
1:2	Average %	100
	Range %	95-105
1:4	Average %	87
	Range %	83-90
1:8	Average %	96
	Range %	93-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.771	2.798	2.785	2.621
50	2.066	2.098	2.082	1.918
25	1.412	1.400	1.406	1.242
12.5	0.841	0.868	0.855	0.691
6.25	0.597	0.575	0.586	0.422
3.12	0.466	0.478	0.472	0.308
1.56	0.318	0.336	0.327	0.163
0	0.163	0.165	0.164

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9NZH6

Applications	ELISA
Research Area	IL37

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10503011	CSB-E16185h	96T	$695	Add to Cart
BHE10503011	CSB-E16185h	5x96T	$3336	Add to Cart
BHE10503011	CSB-E16185h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.