Human Interleukin-36 beta(IL1F8) ELISA kit

Human Interleukin-36 beta(IL1F8) ELISA kit

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Mfr.No.

CSB-EL011619HU

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

interleukin 1 family, member 8 (eta), FIL1, FIL1-(ETA), FIL1H, IL-1F8, IL-1H2, IL1-ETA, IL1H2, MGC126880, MGC126882, IL-1F8 (FIL1-eta), IL1F8 (Canonical product IL-1F8a), Interleukin-1 Superfamily e, family of interleukin 1-eta, interleukin

Recovery

The recovery of human LGALS3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-106
EDTA plasma (n=4)	90	85-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS3BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	91
	Range %	84-98
1:40	Average %	90
	Range %	85-95
1:60	Average %	97
	Range %	92-102
1:160	Average %	102
	Range %	96-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.620	2.645	2.633	2.541
50	2.265	2.366	2.316	2.224
25	1.628	1.673	1.651	1.559
12.5	0.921	0.855	0.888	0.796
6.25	0.571	0.589	0.580	0.488
3.12	0.333	0.355	0.344	0.252
1.56	0.190	0.188	0.189	0.097
0	0.093	0.090	0.092	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9NZH7

Applications	ELISA
Research Area	IL1F8

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503008	CSB-EL011619HU	96T	$695	Add to Cart
BHE10503008	CSB-EL011619HU	5x96T	$3336	Add to Cart
BHE10503008	CSB-EL011619HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.