Human Interleukin-36 alpha(IL1F6) ELISA kit

Human Interleukin-36 alpha(IL1F6) ELISA kit

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Mfr.No.

CSB-EL011617HU

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

interleukin 1 family, member 6 (epsilon), FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), MGC129552, MGC129553, IL-1F6 (FIL-1-epsilon), interleukin 1, epsilon

Recovery

The recovery of mouse GAL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	97	91-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-103
1:2	Average %	90
	Range %	82-99
1:4	Average %	92
	Range %	86-100
1:8	Average %	92
	Range %	89-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.852	2.842	2.847	2.703
10	2.207	2.280	2.244	2.100
5	1.357	1.384	1.371	1.227
2.5	0.841	0.862	0.852	0.708
1.25	0.475	0.458	0.467	0.323
0.625	0.283	0.297	0.290	0.146
0.312	0.231	0.244	0.238	0.094
0	0.142	0.146	0.144

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9UHA7

Applications	ELISA
Research Area	IL36A

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503007	CSB-EL011617HU	96T	$695	Add to Cart
BHE10503007	CSB-EL011617HU	5x96T	$3336	Add to Cart
BHE10503007	CSB-EL011617HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.