Human Interleukin 32 - IL-32 ELISA Kit

Mfr.No.

E0048Hu

Sensitivity

0.027ng/L

Detection Range

0.05-200ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of interleukin 32 (also known as IL 32) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IL 32;IL32;IL-32;Interleukin-32;Natural killer cells protein 4;Tumor necrosis factor alpha-inducing factor

Molecular Weight

26676

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0048Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	86.8	2.82	3.2
Intra-Assay	2	18	65	2.73	4.2
Intra-Assay	3	18	29.7	1.06	3.6

Background

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.

Protocol

These standard curves of E0048Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/L	2.143	2.048	1.993
	1.953
60ng/L	0.963	1.066	1.011
	1.168
30ng/L	0.528	0.487	0.433
	0.447
15ng/L	0.226	0.22	0.166
	0.215
7.5ng/L	0.127	0.139	0.084
	0.151
0ng/L	0.054	0.054	0
	0.055

Gene ID	9235
UniProt ID	P24001

Research Area	Immunology

Storage	2-8°C

Reference	Association between Interleukin-32 and Interleukin-17A Single Nucleotide Polymorphisms and Serum Levels with Polycystic Ovary Syndrome Journal: Iran J Allergy Asthma Immunol. 2019 Feb;18(1):91-99.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101783	E0048Hu	96T	$458		Add to Cart
BHE12101783	E0048Hu	48T	$320		Add to Cart

Human Interleukin 32 - IL-32 ELISA Kit

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