Human Interleukin 31 - IL-31 ELISA Kit

Mfr.No.

E3254Hu

Sensitivity

1.2ng/L

Detection Range

2-600ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Interleukin 31 (also known as IL-31) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IL 31;IL31;IL-31;Interleukin-31

Molecular Weight

18205

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3254Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	88.4	4.8	5.4
Intra-Assay	2	18	141.1	3.33	2.4
Intra-Assay	3	18	68.1	3.17	4.7

Background

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR (PubMed: 15184896). May function in skin immunity (PubMed: 15184896). Enhances myeloid progenitor cell survival in vitro (By similarity). Induces RETNLA and serum amyloid A protein expression in macrophages (By similarity).

Protocol

These standard curves of E3254Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	2.599	2.586	2.53
	2.572
160ng/L	1.561	1.463	1.408
	1.365
80ng/L	0.837	0.786	0.731
	0.736
40ng/L	0.481	0.432	0.377
	0.383
20ng/L	0.285	0.236	0.181
	0.187
0ng/L	0.051	0.055	0
	0.059

Gene ID	386653
UniProt ID	Q6EBC2

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104783	E3254Hu	48T	$320		Add to Cart
BHE12104783	E3254Hu	96T	$458		Add to Cart

Human Interleukin 31 - IL-31 ELISA Kit

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