Human Interleukin 3 - IL-3 ELISA Kit

Mfr.No.

E0093Hu

Sensitivity

1.02pg/ml

Detection Range

2-600pg/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Interleukin 3 (also known as IL-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.>

Alternative Names

Hematopoietic growth factor;IL 3;IL3;IL-3;Interleukin-3;Mast cell growth factor;MCGF;Multipotential colony-stimulating factor;P-cell-stimulating factor;

Molecular Weight

17233

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0093Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	34.7	2.16	6.2
Intra-Assay	2	18	212.5	7.45	3.5
Intra-Assay	3	18	144.1	7.26	5

Background

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Protocol

These standard curves of E0093Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320pg/ml	2.51	2.481	2.421
	2.453
160pg/ml	1.335	1.328	1.268
	1.322
80pg/ml	0.842	0.789	0.728
	0.736
40pg/ml	0.488	0.505	0.444
	0.521
20pg/ml	0.265	0.253	0.193
	0.241
0pg/ml	0.059	0.06	0
	0.062

Gene ID	3562
UniProt ID	P08700

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101828	E0093Hu	96T	$458		Add to Cart
BHE12101828	E0093Hu	48T	$320		Add to Cart

Human Interleukin 3 - IL-3 ELISA Kit

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