Human Interleukin-28 Receptor Subunit Alpha

Mfr.No.

E6452Hu

Description

This sandwich kit is for the accurate quantitative detection of human Interleukin-28 Receptor Subunit Alpha (also known as Il28RA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD25;IL 2Ra;IL-2 receptor subunit alpha;IL2R alpha;IL-2R subunit alpha;IL2RA;IL2-RA;IL-2-RA;Interleukin-2 receptor subunit alpha;p55;TAC antigen

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

30819

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.86ng/L

Background

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Gene ID	3559
UniProt ID	P01589
Antigen	IL2RA

Research Area

Immunology

Protocol

These standard curves of E6452Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.402	2.291	2.236
	2.179
800ng/L	1.328	1.4	1.345
	1.472
400ng/L	0.89	0.876	0.821
	0.861
200ng/L	0.511	0.479	0.424
	0.446
100ng/L	0.269	0.273	0.218
	0.276
0ng/L	0.071	0.055	0
	0.039

Precision

We measured random samples of E6452Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	697.4	23.45	3.4
Intra-Assay	2	18	414.9	26.81	6.5
Intra-Assay	3	18	294.9	14.57	4.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107814	E6452Hu	48T	$320		Add to Cart
BHE12107814	E6452Hu	96T	$458		Add to Cart

Human Interleukin-28 Receptor Subunit Alpha - IL28RA ELISA Kit

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