Human Interleukin 1alpha - IL-1A ELISA Kit

Mfr.No.

E0095Hu

Sensitivity

0.54pg/ml

Detection Range

1-400pg/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Interleukin 1 Alpha (also known as IL-1A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Hematopoietin-1;IL 1 alpha;IL 1A;IL-1 alpha;IL1A;Interleukin-1 alpha

Molecular Weight

30607

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0095Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	142.2	4.62	3.2
Intra-Assay	2	18	89.3	3.03	3.4
Intra-Assay	3	18	63.9	3.27	5.1

Background

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Protocol

These standard curves of E0095Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240pg/ml	2.416	2.409	2.371
	2.402
120pg/ml	1.274	1.343	1.305
	1.412
60pg/ml	0.589	0.595	0.557
	0.601
30pg/ml	0.345	0.39	0.352
	0.435
15pg/ml	0.164	0.176	0.139
	0.189
0pg/ml	0.03	0.038	0
	0.046

Gene ID	3552
UniProt ID	P01583

Research Area	Immunology; Cancer

Storage	2-8°C

Reference	Evaluation of periodontal status and cytokine response in children with familial Mediterranean fever or systemic juvenile idiopathic arthritis Journal: Clin Oral Investig. 2022 Oct 5 Effect of Yoga Therapy on Disease Activity, Inflammatory Markers, and Heart Rate Variability in Patients with Rheumatoid Arthritis Journal: J Altern Complement Med. 2020 Jun;26(6):501-507

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101830	E0095Hu	96T	$458		Add to Cart
BHE12101830	E0095Hu	48T	$320		Add to Cart

Human Interleukin 1alpha - IL-1A ELISA Kit

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