Human Interleukin-15 Receptor Subunit Alpha

Mfr.No.

E5509Hu

Alternative Names

CD antigen CD215;IL 15RA;IL-15 receptor subunit alpha;IL15RA;IL-15RA;IL-15R-alpha;Interleukin-15 receptor subunit alpha;sIL-15 receptor subunit alpha;sIL-15RA;sIL-15R-alpha;Soluble interleukin-15 receptor subunit alpha

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

28233

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.92ng/L

Background

High-affinity receptor for interleukin-15 (PubMed: 8530383). Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells (By similarity). In neutrophils, binds and activates kinase SYK in response to IL15 stimulation (PubMed: 15123770). In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner (PubMed: 15123770). Expression of different isoforms may alter or interfere with signal transduction (PubMed: 10480910). (Isoform 5): Does not bind IL15. (Isoform 6): Does not bind IL15. (Isoform 7): Does not bind IL15. (Isoform 8): Does not bind IL15.

Gene ID	3601
UniProt ID	Q13261
Antigen	IL15RA

Research Area

Immunology

Protocol

These standard curves of E5509Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.124	2.25	2.192
	2.377
1200ng/L	1.415	1.418	1.359
	1.421
600ng/L	0.888	0.824	0.765
	0.759
300ng/L	0.474	0.451	0.393
	0.429
150ng/L	0.25	0.272	0.213
	0.294
0ng/L	0.077	0.059	0
	0.04

Precision

We measured random samples of E5509Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	255.2	13.8	5.4
Intra-Assay	2	18	1467.1	52.94	3.6
Intra-Assay	3	18	593.6	27.58	4.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106871	E5509Hu	48T	$320		Add to Cart
BHE12106871	E5509Hu	96T	$458		Add to Cart

Human Interleukin-15 Receptor Subunit Alpha - IL15RA ELISA Kit

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