Human Interleukin 15 - IL-15 ELISA Kit

Mfr.No.

E0097Hu

Description

This sandwich kit is for the accurate quantitative detection of human Interleukin 15 (also known as IL-15) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IL 15;IL15;IL-15;Interleukin-15

Reactivity

Human

Detection Range

5-1500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

18086

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.98ng/L

Background

Cytokine that stimulates the proliferation of T-lymphocytes (PubMed: 8178155). Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA (PubMed: 8178155). In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation (PubMed: 15123770).

Gene ID	3600
UniProt ID	P40933
Antigen	IL15

Research Area

Immunology

Protocol

These standard curves of E0097Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	1.903	1.806	1.768
	1.71
400ng/L	1.037	0.964	0.926
	0.891
200ng/L	0.483	0.551	0.512
	0.618
100ng/L	0.144	0.155	0.117
	0.166
50ng/L	0.082	0.073	0.034
	0.063
0ng/L	0.026	0.038	0
	0.051

Precision

We measured random samples of E0097Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	745	26.51	3.6
Intra-Assay	2	18	156.1	6.02	3.9
Intra-Assay	3	18	455	24.25	5.3

Storage	2-8°C

Reference	Serum Inflammatory and Oxidative Stress Markers in Patients with Vitiligo Journal: J Clin Med. 2023 Sep 9;12(18):5861.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101832	E0097Hu	96T	$458		Add to Cart
BHE12101832	E0097Hu	48T	$320		Add to Cart

Human Interleukin 15 - IL-15 ELISA Kit

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