Human Interleukin-12 Subunit Alpha

Mfr.No.

E5508Hu

Sensitivity

4.67ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CLMF p35;Cytotoxic lymphocyte maturation factor 35 kDa subunit;IL 12A;IL 12alpha;IL-12 subunit p35;IL12A;IL-12A;Interleukin-12 subunit alpha;NK cell stimulatory factor chain 1;NKSF1

Molecular Weight

24874

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5508Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	787	41.04	5.2
Intra-Assay	2	18	486.2	17.15	3.5
Intra-Assay	3	18	174.2	6.39	3.7

Background

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Protocol

These standard curves of E5508Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	1.946	1.982	1.931
	2.017
600ng/L	1.409	1.432	1.382
	1.456
300ng/L	0.626	0.653	0.602
	0.68
150ng/L	0.386	0.372	0.321
	0.358
75ng/L	0.175	0.195	0.144
	0.215
0ng/L	0.053	0.051	0
	0.049

Gene ID	3592
UniProt ID	P29459

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106870	E5508Hu	48T	$320		Add to Cart
BHE12106870	E5508Hu	96T	$458		Add to Cart

Human Interleukin-12 Subunit Alpha - IL12A ELISA Kit

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