Human Interleukin 12 Receptor Subunit Beta-1

Mfr.No.

E3978Hu

Description

This sandwich kit is for the accurate quantitative detection of human Interleukin 12 Receptor Subunit Beta-1 (also known as IL12RB1/IL12R/IL12RB) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD212;IL 12RB1;IL-12 receptor beta component;IL-12 receptor subunit beta-1;IL-12R subunit beta-1;IL12RB1;IL-12RB1;IL-12R-beta-1;Interleukin-12 receptor subunit beta-1;

Reactivity

Human

Detection Range

0.05-15ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

73109

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.01ng/ml

Background

Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

Gene ID	3594
UniProt ID	P42701
Antigen	IL12RB1

Research Area

Others

Protocol

These standard curves of E3978Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.156	2.145	2.111
	2.134
4ng/ml	1.749	1.723	1.689
	1.696
2ng/ml	1.128	1.18	1.146
	1.232
1ng/ml	0.667	0.688	0.654
	0.709
0.5ng/ml	0.352	0.359	0.326
	0.367
0ng/ml	0.035	0.034	0
	0.033

Precision

We measured random samples of E3978Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3	0.16	5.4
Intra-Assay	2	18	0.6	0.03	5.3
Intra-Assay	3	18	1.4	0.09	6.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105411	E3978Hu	48T	$320		Add to Cart
BHE12105411	E3978Hu	96T	$458		Add to Cart

Human Interleukin 12 Receptor Subunit Beta-1 - IL12RB1 ELISA Kit

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