Human Interferon-induced Gtp-binding Protein Mx1

Mfr.No.

E5499Hu

Sensitivity

5.23ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

IFI-78K;Interferon-induced GTP-binding protein Mx1;Interferon-induced GTP-binding protein Mx1, N-terminally processed;Interferon-induced protein p78;Interferon-regulated resistance GTP-binding protein MxA;MX 1;MX1;Myxoma resistance protein 1;Myxovirus resistance protein 1;

Molecular Weight

75520

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5499Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	845.5	44.67	5.3
Intra-Assay	2	18	204.6	11.52	5.6
Intra-Assay	3	18	361.6	12.62	3.5

Background

Interferon-induced dynamin-like GTPase with antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. May also antagonize reoviridae and asfarviridae replication. Inhibits thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. Inhibits La Crosse virus (LACV) replication by sequestering viral nucleoprotein in perinuclear complexes, preventing genome amplification, budding, and egress. Inhibits influenza A virus (IAV) replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles. Enhances ER stress-mediated cell death after influenza virus infection. May regulate the calcium Channel activity of TRPCs.

Protocol

These standard curves of E5499Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.224	2.236	2.174
	2.247
600ng/L	1.51	1.498	1.437
	1.486
300ng/L	0.792	0.793	0.732
	0.794
150ng/L	0.445	0.455	0.394
	0.466
75ng/L	0.244	0.254	0.193
	0.265
0ng/L	0.059	0.061	0
	0.063

Gene ID	4599
UniProt ID	P20591

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106861	E5499Hu	48T	$320		Add to Cart
BHE12106861	E5499Hu	96T	$458		Add to Cart

Human Interferon-induced Gtp-binding Protein Mx1 - MX1 ELISA Kit

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