Human Interferon gamma (IFN-gamma) ELISA Kit

Mfr.No.

CSB-E04577h

Reactivity

Human

Detection Range

0.312 ng/ml-20 ng/ml

Sample Type

Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human S100A7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-101
EDTA plasma (n=4)	86	81-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	92
	Range %	89-96
1:4	Average %	98
	Range %	94-102
1:8	Average %	91
	Range %	82-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.078 ng/ml

Limit Of Detection

450 nm

UniProt ID	P01579
Antigen	interferon, gamma

Applications

ELISA

Research Area

IFNG

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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