Human Interferon Alpha-21 - IFNA21 ELISA Kit

Mfr.No.

E5494Hu

Sensitivity

5.46ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

IFNA 21;IFNA21;IFN-alpha-21;Interferon alpha-21;Interferon alpha-F;LeIF F

Molecular Weight

21741

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5494Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	776	35.33	4.6
Intra-Assay	2	18	247.8	16.11	6.5
Intra-Assay	3	18	123.9	9.85	7.9

Background

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.

Protocol

These standard curves of E5494Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1280ng/L	2.167	2.238	2.192
	2.309
640ng/L	1.644	1.578	1.532
	1.512
320ng/L	0.957	1.052	1.006
	1.147
160ng/L	0.727	0.715	0.669
	0.702
80ng/L	0.447	0.435	0.389
	0.423
0ng/L	0.051	0.046	0
	0.041

Gene ID	3452
UniProt ID	P01568

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106856	E5494Hu	48T	$320		Add to Cart
BHE12106856	E5494Hu	96T	$458		Add to Cart

Human Interferon Alpha-21 - IFNA21 ELISA Kit

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