Human Inter-alpha-trypsin inhibitor heavy chain H5(ITIH5) ELISA kit

Mfr.No.

CSB-EL011898HU

Alternative Names

inter-alpha (globulin) inhibitor H5, DKFZp686F0145, MGC10848, pp14776, inter-alpha trypsin inhibitor heavy chain precursor 5

Reactivity

Human

Detection Range

1.56 ng/mL-100 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human LAMA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	95-107
EDTA plasma (n=4)	104	98-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LAMA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	97
	Range %	89-101
1:10	Average %	106
	Range %	100-110
1:20	Average %	93
	Range %	86-96
1:40	Average %	107
	Range %	92-111

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.39 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q86UX2
Antigen	inter-alpha (globulin) inhibitor H5

Applications

ELISA

Research Area

ITIH5

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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