Human Integrin alphaM,ITG/CD11b ELISA Kit

Human Integrin alphaM,ITG/CD11b ELISA Kit

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Mfr.No.

CSB-E11638h

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

integrin, alpha M (complement component 3 receptor 3 subunit), CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6, antigen CD11b (p170), integrin alpha M, macrophage antigen alpha polypeptide, neutrophil adherence receptor alpha-M subunit

Recovery

The recovery of human CK20 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	96	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CK20 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-108
1:2	Average %	86
	Range %	80-92
1:4	Average %	95
	Range %	90-100
1:8	Average %	94
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.766	2.868	2.817	2.673
20	2.101	1.997	2.049	1.905
10	1.172	1.244	1.208	1.064
5	0.725	0.732	0.729	0.585
2.5	0.363	0.387	0.375	0.231
1.25	0.266	0.284	0.275	0.131
0.625	0.228	0.246	0.237	0.093
0	0.144	0.143	0.144	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P11215

Applications	ELISA
Research Area	ITGAM

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502894	CSB-E11638h	96T	$695	Add to Cart
BHE10502894	CSB-E11638h	5x96T	$3336	Add to Cart
BHE10502894	CSB-E11638h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.