Human Integrin alpha-IIb(ITGA2B) ELISA kit

Human Integrin alpha-IIb(ITGA2B) ELISA kit

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Mfr.No.

CSB-EL011865HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41), CD41, CD41B, GP2B, GPIIb, GTA, HPA3, integrin alpha 2b, platelet fibrinogen receptor, alpha subunit, platelet-specific antigen BAK

Recovery

The recovery of human CK-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-102
EDTA plasma (n=4)	97	92-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CK-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	92
	Range %	88-99
1:4	Average %	97
	Range %	92-106
1:8	Average %	94
	Range %	86-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
15	1.932	1.958	1.945	1.898
7.5	1.318	1.357	1.338	1.291
3.75	0.786	0.798	0.792	0.745
1.25	0.375	0.362	0.369	0.322
0.625	0.216	0.223	0.220	0.173
0	0.046	0.047	0.047	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P08514

Applications	ELISA
Research Area	ITGA2B

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502885	CSB-EL011865HU	96T	$695	Add to Cart
BHE10502885	CSB-EL011865HU	5x96T	$3336	Add to Cart
BHE10502885	CSB-EL011865HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<10%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.