Human Integrin alpha-4(ITGA4/CD49D)ELISA kit

Mfr.No.

CSB-E17545h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Alternative Names

integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor), CD49D, IA4, MGC90518, 269C wild type, antigen CD49D, alpha-4 subunit of VLA-4 receptor, integrin alpha 4, integrin alpha-4 subunit, very late activation protein 4 receptor, alpha 4 subunit

Recovery

The recovery of human CX3CR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	97	92-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CX3CR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	92
	Range %	88-97
1:4	Average %	84
	Range %	80-88
1:8	Average %	101
	Range %	95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.764	2.647	2.706	2.549
1000	2.006	2.018	2.012	1.855
500	1.295	1.238	1.267	1.110
250	0.731	0.753	0.742	0.585
125	0.498	0.512	0.505	0.348
62.5	0.327	0.341	0.334	0.177
31.25	0.243	0.239	0.241	0.084
0	0.159	0.154	0.157	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	P13612

Applications	ELISA
Research Area	ITGA4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502883	CSB-E17545h	96T	$790	Add to Cart
BHE10502883	CSB-E17545h	5x96T	$3792	Add to Cart
BHE10502883	CSB-E17545h	10x96T	$7505	Add to Cart

Human Integrin alpha-4(ITGA4/CD49D)ELISA kit

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