Human Insulin Receptor Substrate 2

Mfr.No.

E0035Hu

Sensitivity

0.23ng/L

Detection Range

0.5-200ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Insulin Receptor Substrate 2 (also known as IRS-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Insulin receptor substrate 2;IRS2;IRS-2

Molecular Weight

137334

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0035Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	97.9	2.55	2.61
Intra-Assay	2	18	67.13	2.89	4.3
Intra-Assay	3	18	35.41	1.88	5.31

Background

May mediate the control of various cellular processes by insulin.

Protocol

These standard curves of E0035Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/L	1.868	1.994	1.946
	2.12
60ng/L	1.097	1.031	0.983
	0.965
30ng/L	0.587	0.501	0.453
	0.416
15ng/L	0.207	0.223	0.174
	0.239
7.5ng/L	0.149	0.153	0.105
	0.157
0ng/L	0.061	0.048	0
	0.035

Gene ID	8660
UniProt ID	Q9Y4H2

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101771	E0035Hu	48T	$320		Add to Cart
BHE12101771	E0035Hu	96T	$458		Add to Cart

Human Insulin Receptor Substrate 2 - IRS-2 ELISA Kit

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