Human Insulin Receptor Substrate 1

Mfr.No.

E3129Hu

Sensitivity

0.09ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Insulin receptor substrate 1;IRS 1;IRS1;IRS-1

Molecular Weight

131591

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3129Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	17.8	1.11	6.3
Intra-Assay	2	18	6	0.33	5.5
Intra-Assay	3	18	3.5	0.19	5.3

Background

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

Protocol

These standard curves of E3129Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.376	2.305	2.265
	2.233
20ng/ml	1.916	1.923	1.884
	1.93
10ng/ml	1.241	1.168	1.129
	1.095
5ng/ml	0.673	0.669	0.63
	0.665
2.5ng/ml	0.377	0.408	0.369
	0.439
0ng/ml	0.028	0.039	0
	0.051

Gene ID	3667
UniProt ID	P35568

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104661	E3129Hu	48T	$320		Add to Cart
BHE12104661	E3129Hu	96T	$458		Add to Cart

Human Insulin Receptor Substrate 1 - IRS-1 ELISA Kit

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