Human Insulin-like peptide INSL5(INSL5) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL011749HU

Sensitivity

5 ng/mL

Detection Range

8 ng/mL-140 ng/mL

Reactivity

Human

Assay Duration

1-5h

Alternative Names

insulin-like 5, MGC126695, MGC126697, PRO182, UNQ156, insulin-like peptide 5

Recovery

The recovery of mouse LN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-102
1:2	Average %	87
	Range %	84-90
1:4	Average %	91
	Range %	88-96
1:8	Average %	101
	Range %	97-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

UniProt ID	Q9Y5Q6

Applications	ELISA
Research Area	INSL5

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Circulating insulin-like peptide 5 levels and its association with metabolic and hormonal parameters in women with polycystic ovary syndrome M. Bicer.et al,Journal of Endocrinological Investigation,June 28

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