Human Insulin-like Growth Factors Binding Protein 3

Mfr.No.

E0391Hu

Sensitivity

0.026ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Insulin-like Growth Factors Binding Protein 3 (also known as IGFBP-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IBP-3;IGF-binding protein 3;IGFBP 3;IGFBP3;IGFBP-3;Insulin-like growth factor-binding protein 3

Molecular Weight

31674

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0391Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.1	0.24	3
Intra-Assay	2	18	5.1	0.21	4.2
Intra-Assay	3	18	4.2	0.22	5.2

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Protocol

These standard curves of E0391Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	1.771	1.833	1.786
	1.895
6ng/ml	0.898	1.008	0.961
	1.117
3ng/ml	0.42	0.495	0.449
	0.571
1.5ng/ml	0.219	0.236	0.19
	0.254
0.75ng/ml	0.095	0.084	0.038
	0.073
0ng/ml	0.031	0.046	0
	0.062

Gene ID	3486
UniProt ID	P17936

Research Area	Cell Biology; Signal Transduction; Cardiovascular; Metabolism; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102087	E0391Hu	96T	$458		Add to Cart
BHE12102087	E0391Hu	48T	$320		Add to Cart

Human Insulin-like Growth Factors Binding Protein 3 - IGFBP-3 ELISA Kit

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