Human Insulin-like Growth Factors Binding Protein 1

Mfr.No.

E0411Hu

Description

This sandwich kit is for the accurate quantitative detection of human Insulin-like Growth Factors Binding Protein 1 (also known as IGFBP-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IBP-1;IGF-binding protein 1;IGFBP 1;IGFBP1;IGFBP-1;Insulin-like growth factor-binding protein 1;Placental protein 12;PP12

Reactivity

Human

Detection Range

5-2000ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

27904

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.12ng/ml

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

Gene ID	3484
UniProt ID	P08833
Antigen	IGFBP1

Research Area

Signal Transduction

Protocol

These standard curves of E0411Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/ml	2.207	2.261	2.207
	2.316
600ng/ml	1.812	1.885	1.831
	1.957
300ng/ml	1.121	1.19	1.136
	1.258
150ng/ml	0.618	0.648	0.594
	0.678
75ng/ml	0.385	0.365	0.311
	0.346
0ng/ml	0.053	0.054	0
	0.055

Precision

We measured random samples of E0411Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	228.3	12.6	5.5
Intra-Assay	2	18	428.3	15.22	3.6
Intra-Assay	3	18	124.4	6.21	5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102107	E0411Hu	96T	$458		Add to Cart
BHE12102107	E0411Hu	48T	$320		Add to Cart

Human Insulin-like Growth Factors Binding Protein 1 - IGFBP1 ELISA Kit

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