Human insulin-like growth factors binding protein 1,IGFBP-1 ELISA Kit

Human insulin-like growth factors binding protein 1,IGFBP-1 ELISA Kit

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Mfr.No.

CSB-E04586h

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Human

Assay Duration

1-5h

Alternative Names

insulin-like growth factor binding protein 1, AFBP ELISA Kit; Alpha pregnancy associated endometrial globulin ELISA Kit; Amniotic fluid binding protein ELISA Kit; Binding protein 25 ELISA Kit; Binding protein 26 ELISA Kit; Binding protein 28 ELISA Kit; Growth hormone independent binding protein ELISA Kit; hIGFBP 1 ELISA Kit; hIGFBP1 ELISA Kit; IBP 1 ELISA Kit; IBP-1 ELISA Kit; IBP1 ELISA Kit; IBP1_HUMAN ELISA Kit; IGF binding protein 1 ELISA Kit; IGF BP25 ELISA Kit; IGF-binding protein 1 ELISA Kit; IGFBP 1 ELISA Kit; IGFBP-1 ELISA Kit; IGFBP1 ELISA Kit; Insulin like growth factor binding protein 1 ELISA Kit; Insulin-like growth factor-binding protein 1 ELISA Kit; Placental protein 12 ELISA Kit; PP 12 ELISA Kit; PP12 ELISA Kit

Recovery

The recovery of human sAPP? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	85-97
EDTA plasma (n=4)	97	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sAPP? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-94
1:2	Average %	101
	Range %	90-105
1:4	Average %	91
	Range %	85-94
1:8	Average %	93
	Range %	85-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	1.822	1.902	1.862	1.729
400	1.454	1.351	1.403	1.270
200	0.859	0.901	0.880	0.747
100	0.537	0.548	0.543	0.410
50	0.339	0.345	0.342	0.209
25	0.234	0.222	0.228	0.095
12.5	0.166	0.172	0.169	0.036
0	0.132	0.134	0.133

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P08833

Applications	ELISA
Research Area	IGFBP1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	YAP mediates humanDecidualization of the uterine endometrial stromal cells Hengxi Chen.et al,Placenta,May 17

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SKU	Supplier No.	Size	Your price
BHE10502872	CSB-E04586h	96T	$495	Add to Cart
BHE10502872	CSB-E04586h	5x96T	$2376	Add to Cart
BHE10502872	CSB-E04586h	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.