Human insulin-like growth factor binding protein 5,IGFBP-5 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13263h

Alternative Names

insulin-like growth factor binding protein 5, IBP5,

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of fish CYP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	88-95
EDTA plasma (n=4)	94	91-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish CYP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	100
	Range %	92-107
1:4	Average %	85
	Range %	80-91
1:8	Average %	88
	Range %	83-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	P24593
Antigen	insulin-like growth factor binding protein 5

Applications

ELISA

Research Area

IGFBP5

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10502863	CSB-E13263h	96T	$468	Add to Cart
BHE10502863	CSB-E13263h	5x96T	$2246.40	Add to Cart
BHE10502863	CSB-E13263h	10x96T	$4446	Add to Cart