Human Insulin Like Growth Factor Binding Protein 4

Mfr.No.

E6689Hu

Alternative Names

IBP-4;IGF-binding protein 4;IGFBP 4;IGFBP4;IGFBP-4;Insulin-like growth factor-binding protein 4

Reactivity

Human

Detection Range

1-400ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

27934

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.53ng/ml

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Gene ID	3487
UniProt ID	P22692
Antigen	IGFBP4

Research Area

Signal Transduction

Protocol

These standard curves of E6689Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.224	2.168	2.137
	2.113
120ng/ml	1.259	1.299	1.268
	1.34
60ng/ml	0.704	0.66	0.628
	0.615
30ng/ml	0.345	0.393	0.362
	0.442
15ng/ml	0.189	0.172	0.14
	0.154
0ng/ml	0.018	0.031	0
	0.045

Precision

We measured random samples of E6689Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	64.8	2.86	4.4
Intra-Assay	2	18	121.2	4.4	3.6
Intra-Assay	3	18	39.1	2.27	5.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115217	E6689Hu	48T	$320		Add to Cart
BHE12115217	E6689Hu	96T	$458		Add to Cart

Human Insulin Like Growth Factor Binding Protein 4 - IGFBP4 ELISA Kit

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