Human Insulin-like growth factor 2 mRNA-binding protein 3(IGF2BP3) ELISA kit

Human Insulin-like growth factor 2 mRNA-binding protein 3(IGF2BP3) ELISA kit

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Mfr.No.

CSB-EL011092HU

Alternative Names

insulin-like growth factor 2 mRNA binding protein 3, CT98, DKFZp686F1078, IMP-3, IMP3, KOC1, VICKZ3, IGF II mRNA binding protein 3, IGF-II mRNA-binding protein 3, KH domain containing protein overexpressed in cancer, cancer/testis antigen 98

Reactivity

Human

Detection Range

37.5 pg/mL-2400 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-95
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	86
	Range %	80-92
1:200	Average %	96
	Range %	92-100
1:400	Average %	100
	Range %	93-110
1:800	Average %	91
	Range %	85-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mll	OD1	OD2	Average	Corrected
40	2.439	2.252	2.346	2.241
20	1.857	1.760	1.809	1.704
10	1.340	1.304	1.322	1.217
5	0.903	0.890	0.897	0.792
2.5	0.515	0.504	0.510	0.405
1.25	0.342	0.319	0.331	0.226
0.625	0.213	0.203	0.208	0.103
0	0.109	0.101	0.105	?

Species

Homo sapiens

Sensitivity

9.37 pg/mL

Limit Of Detection

450 nm

UniProt ID	O00425
Antigen	insulin-like growth factor 2 mRNA binding protein 3

Applications

ELISA

Research Area

IGF2BP3

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502862	CSB-EL011092HU	96T	$695	Add to Cart
BHE10502862	CSB-EL011092HU	5x96T	$3336	Add to Cart
BHE10502862	CSB-EL011092HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.