Human Insulin-like 3 - INSL3 ELISA Kit

Mfr.No.

E3046Hu

Description

This sandwich kit is for the accurate quantitative detection of human Insulin Like Protein 3 (also known as INSL3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

INSL 3;INSL3;Insulin-like 3;Insulin-like 3 A chain;Insulin-like 3 B chain;Leydig Insulin-like Peptide;Ley-I-L;Relaxin-like factor

Reactivity

Human

Detection Range

0.2-60ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

14502

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.11ng/ml

Background

Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.

Gene ID	3640
UniProt ID	P51460
Antigen	INSL3

Research Area

Signal Transduction

Protocol

These standard curves of E3046Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.163	2.151	2.096
	2.138
20ng/ml	1.181	1.263	1.209
	1.346
10ng/ml	0.834	0.73	0.676
	0.627
5ng/ml	0.529	0.514	0.459
	0.498
2.5ng/ml	0.195	0.188	0.134
	0.181
0ng/ml	0.059	0.054	0
	0.05

Precision

We measured random samples of E3046Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6	0.45	7.5
Intra-Assay	2	18	22.7	0.91	4
Intra-Assay	3	18	12.8	0.7	5.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104580	E3046Hu	48T	$320		Add to Cart
BHE12104580	E3046Hu	96T	$458		Add to Cart

Human Insulin-like 3 - INSL3 ELISA Kit

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