Human Insulin,INS ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E05069h

Reactivity

Human

Sample Type

Cell culture supernates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human LPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	80-96
EDTA plasma (n=4)	96	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-102
1:200	Average %	94
	Range %	91-98
1:400	Average %	102
	Range %	98-106
1:800	Average %	87
	Range %	82-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

UniProt ID	P01308
Antigen	Insulin

Applications

ELISA

Research Area

INS

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502859	CSB-E05069h	96T	$450	Add to Cart
BHE10502859	CSB-E05069h	5x96T	$2160	Add to Cart
BHE10502859	CSB-E05069h	10x96T	$4275	Add to Cart