Human Insulin - INS ELISA Kit

Mfr.No.

E0010Hu

Sensitivity

0.11mIU/L

Detection Range

0.2-60mIU/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

INS;Insulin A chain;Insulin B chain

Molecular Weight

11981

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0010Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.8	0.52	5.9
Intra-Assay	2	18	30.5	1.19	3.9
Intra-Assay	3	18	13.3	0.7	5.2

Background

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Protocol

These standard curves of E0010Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40mIU/L	2.28	2.194	2.152
	2.109
20mIU/L	1.174	1.286	1.244
	1.398
10mIU/L	0.839	0.741	0.7
	0.644
5mIU/L	0.399	0.438	0.396
	0.477
2.5mIU/L	0.235	0.212	0.17
	0.188
0mIU/L	0.026	0.042	0
	0.058

Gene ID	3630
UniProt ID	P01308

Research Area	Metabolism

Storage	2-8°C

Reference	Dietary energy density, metabolic parameters, and blood pressure in a sample of adults with obesity Journal: BMC Endocr Disord. 2023 Jan 5;23(1):3. Evaluating the association between the Mediterranean-DASH Intervention for Neurodegenerative Delay (MIND) diet, mental health, and cardio-metabolic risk factors among individuals with obesity Journal: BMC Endocr Disord. 2023 Feb 2;23(1):29. Calcium and vitamin D3 combinations improve fatty liver disease through AMPK-independent mechanisms Journal: Eur J Nutr. 2018 Mar;57(2):731-740. Asprosin in umbilical cord of newborns and maternal blood of gestational diabetes, preeclampsia, severe preeclampsia, intrauterine growth retardation and macrosemic fetus Journal: Peptides. 2019 Oct;120:170132. Bitter gourd reduces elevated fasting plasma glucose levels in an intervention study among prediabetics in Tanzania Journal: J Ethnopharmacol. 2018 Apr 24;216:1-7. Dietary Antioxidant Quality Score (DAQS), serum lipids, markers of glucose homeostasis, blood pressure and anthropometric features among apparently metabolically healthy obese adults in two metropolises of Iran (Tabriz and Tehran): a cross-sectional study Journal: BMC Endocr Disord. 2023 Jul 21;23(1):157. Dietary Antioxidant Quality Score (DAQS), serum lipids, markers of glucose homeostasis, blood pressure and anthropometric features among apparently metabolically healthy obese adults in two metropolises of Iran (Tabriz and Tehran): a cross-sectional study Journal: BMC Endocr Disord. 2023 Jul 21;23(1):157.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115203	E0010Hu	96T	$458		Add to Cart
BHE12115203	E0010Hu	48T	$320		Add to Cart

Human Insulin - INS ELISA Kit

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