Human insulin autoantibodies,IAA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09205h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

insulin autoantibodies,IAA

Recovery

The recovery of human DEFA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-102
EDTA plasma (n=4)	90	86-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-94
1:2	Average %	84
	Range %	80-88
1:4	Average %	93
	Range %	89-97
1:8	Average %	95
	Range %	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IAA

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502853	CSB-E09205h	96T	$495	Add to Cart
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BHE10502853	CSB-E09205h	10x96T	$4702.50	Add to Cart