Matrices listed below were spiked with certain level of INSM2 and the recovery rates were calculated by comparing the measured value to the expected amount of INSM2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
96-102
98
EDTA plasma(n=5)
97-103
100
heparin plasma(n=5)
95-103
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of INSM2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
95-102%
94-103%
89-100%
EDTA plasma(n=5)
91-96%
89-97%
90-100%
heparin plasma(n=5)
90-99%
90-98%
90-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q96T92
Applications
ELISA
Applications Notes
INSM2 ELISA Kit allows for the in vitro quantitative determination of INSM2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human INSM2 (Insulinoma-associated protein 2) ELISA Kit