Human Inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) ELISA kit

Human Inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) ELISA kit

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Mfr.No.

CSB-E17855h

Sensitivity

5.8 pg/mL

Detection Range

23.5 pg/mL-1500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

IMP (inosine monophosphate) dehydrogenase 2, hCG_2002013, IMPD2, IMPDH-II, IMP oxireductase 2, inosine 5 phosphate dehydrogenase 2, inosine monophosphate dehydrogenase 2, inosine monophosphate dehydrogenase type II

Recovery

The recovery of rat CTX-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-102
EDTA plasma (n=4)	86	82-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	92
	Range %	88-96
1:4	Average %	98
	Range %	94-102
1:8	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.353	0.354	0.354
750	0.468	0.475	0.472
375	0.616	0.605	0.611
125	1.011	0.995	1.003
25	1.512	1.594	1.553
0	2.013	2.114	2.064

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P12268

Applications	ELISA
Research Area	IMPDH2

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10502849	CSB-E17855h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.